Single-molecule studies of eukaryotic DNA replication

Single-molecule studies of eukaryotic DNA replication

by Anna Barbara Loveland

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DNA replication is a fundamental cellular process. However, the structure and dynamics of the eukaryotic DNA replication machinery remain poorly understood. A soluble extract system prepared from Xenopus eggs recapitulates eukaryotic DNA replication outside of a cell on a variety of DNA templates. This system has been used to reveal many aspects of DNA replication using a variety of ensemble biochemical techniques.

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