Decoding Tumor Complexity

Tumor heterogeneity poses a formidable challenge in oncology, manifesting as genetic, epigenetic, and phenotypic variations within neoplastic tissues that underpin therapeutic resistance and metastatic progression. This abstract delineates an integrative framework leveraging spatial transcriptomics and 3D chromatin conformation capture to unravel and mitigate intratumoral diversity. The primary objective is to dissect the spatiotemporal dynamics of genomic architecture in solid tumors, hypothesizing that aberrant chromatin looping and spatial gene expression patterns drive subclonal evolution. Employing high-resolution spatial RNA sequencing (e.g., Visium platform) alongside Hi-C and derivative techniques, we mapped 3D genomic interactions across patient-derived tumor organoids and biopsy samples from breast and colorectal carcinomas (n=45).