Chromatin dependent microtubule assembly during meiosis

Cell division, or mitosis, is the process whereby one cell divides into two daughter cells and is required for many aspects of life, including growth, immune response, and tissue repair; however, when unregulated, errors can contribute to uncontrolled division and cancerous tumor growth. Thus, understanding the mechanisms of cell division is of critical importance. The process of dividing one cell into 2 daughter cells requires the precise coordination of many forces that operate to drive the equal segregation of the genetic material. Components of the cytoskeleton, such as microtubules, provide a structure to transmit the required forces and are essential for cell division. Thus, understanding the mechanism of microtubule assembly is required to understand how cells divide. The genetic material--known as chromatin--induces the assembly of microtubules during meiosis. However the precise mechanism of how this occurs is unknown. This dissertation identifies novel factors involved in chromatin dependent microtubule assemble. Experiments presented in this dissertation, we find there are multiple factors which simultaneously function in the process, including motor proteins, such as Kinesin-5 which distribute microtubule assembly properties to the spindle poles. Finally and most importantly, we find that microtubule assembly dependent microtubule assembly requires soluble cytosol containing only glycogen, without the large membrane structures--such as golgi, ER, and mitochondria--which reflect light, giving the opportunity for live imaging analysis of microtubule assembly and further biochemical purification for in vitro reconstitution.