Plant Cell Culture Protocols

This second edition of Plant Cell Protocols follows a similar vein as its successful predecessor in providing an updated step=by-step guide to the most common, and applicable techniques and methods for plant tissue and cell culture. A total of 30 chapters, divided into 6 major sections have been included. Topics selected cover from general methodologies, such as culture induction, growth and viability evaluation, and contamination control, to such highly specialized techniques as chloroplast transformation, passing through the laborious process of protoplast isolation and culture. The protocols are currently used in the research programs of the authors, or represent important parts of business projects aimed at the generation of improved plant materials.